|
KMID : 1007520100190030849
|
|
Food Science and Biotechnology
2010 Volume.19 No. 3 p.849 ~ p.855
|
|
|
Molecular Cloning of the Genes for GDP-mannose 4, 6-dehydratase and GDP-L-fucose Synthetase from Bacteroides thetaiotaomicron
|
|
Jang Mi-Hee
Lee Won-Heong
Shin So-Yeon
Han Nam-Soo
Seo Jin-Ho
Kim Myoung-Dong
|
|
|
Abstract
|
|
|
|
Genes encoding GDP-mannose 4, 6-dehydratase (GMD) and GDP-L-fucose synthetase (GFS) were cloned from Bacteroides thetaiotaomicron and overexpressed in recombinant Escherichia coli by constructing isopropyl-β- D-thiogalactopyranoside (IPTG)-inducible expression vectors. GMD and GFS genes from B. thetaiotaomicron were 60 and 45%, respectively, identical to those from E. coli K12 over their entire lengths. An optimum expression condition of 30oC and 0.1 mM IPTG was chosen for maximum soluble expression of B. thetaiotaomicron GMD and GFS in recombinant E. coli BL21(DE3). Functional expression of B. thetaiotaomicron GMD and GFS in recombinant E. coli strains was confirmed by measuring intracellular GDP-L-fucose content.
|
|
|
KEYWORD
|
|
|
GDP-L-fucose, GDP-mannose 4, 6-dehydratase, GDP-L-fucose synthetase, Bacteroides thetaiotaomicron, recombinant Escherichia coli
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|